Fig. 2. Androgen receptor (AR) expression with pro-inflammatory cytokine exposure (TNFα and IL-13) in non-asthmatic and asthmatic human ASM cells (males and females). A) AR expression in non-asthmatic human ASM cells (both male and female) using qRT-PCR analysis; B) AR expression in asthmatic human ASM cells (both male and female) using qRT-PCR analysis. S16 was used as internal control for normalization. C) AR expression in non-asthmatic human ASM cells (both male and female) using western analysis and D) AR expression in asthmatic human ASM cells (both male and female) using western analysis. β-actin was used as internal control for normalization. AR expression is significantly increased during inflammation, especially in female ASM with pronounced changes observed in TNFα exposed asthmatic female ASM. *p<0.05; **p<0.01 and ***p<0.001 vs. vehicle. Data expressed as mean ± SEM. n=5 patients each.